KLOTHO PROTEIN AS A PROGNOSTIC MARKER OF PERIODONTAL TISSUE CONDITION IN ORTHODONTIC TREATMENT WITH FIXED EQUIPMENT IN YOUNG PEOPLE
DOI:
https://doi.org/10.35220/2523-420X/2020.1.11Keywords:
Klotho protein, chronic catarrhal gingivitis, orthodontic treatment, oral fluidAbstract
The Klotho protein has a molecular weight of 130 kDa and consists of 1014 amino acids, has a signal sequence at the N-end and a transmembrane domain with a short cytoplasmic domain at the C-end. The extracellular Klotho do-main consists of 2 internal repeats (KL1 and KL2) of homologous β-glucosidase sequences. The Klotho protein is ex-pressed in many tissues, including the oral fluid.
Purpose of research. Studying the expression of Klotho-protein in the oral fluid in young people in the dynamics of orthodontic treatment with fixed structures.
Research materials and methods. We conducted a study of 44 young people aged 16 to 25 years. All the studied patients were divided into 3 groups, in which they were diagnosed with Chronic catarrhal gingivitis, which occurred against the background of dental anomalies or during orthodontic treatment. The comparison group consisted of young people who did not have orthodontic pathology and inflammatory periodontal diseases and were clinically healthy. Bi-ochemical studies were performed in the patients ' oral fluid. For the study of Klotho protein, oral fluid was collected in the morning on an empty stomach, which was obtained without stimulation by spitting into sterile test tubes.
The results of the study and their discussion. The analysis of digital data on the content of Klotho protein in the oral fluid of patients with chronic catarrhal gingivitis, during which it occurred against the background of HPA, showed that the established values were 26.06 ± 1.33 ng / ml and this is 2.2 times less for data in healthy young people (57.03 ± 2.92 ng / ml). However, attention is drawn to the fact that patients who underwent orthodontic treatment with fixed equipment found a sharp decrease in the content of the studied indicator in the oral fluid.
Conclusions. The expression of the Klotho protein in the oral fluid can be a reliable predictive sign of the devel-opment of inflammatory and destructive complications in periodontal tissues against the background of orthodontic treatment using fixed equipment.
References
Fábián T.K., Fejérdy P., Csermely P. Salivary ge-nomics, transcriptomics and proteomics: The emerging concept of the oral ecosystem and their use in the early diagnosis of can-cer and other diseases. Curr. Genomics. 2008;9:11–21.
Madhwani T., McBain A.J. Compositional modifica-tion of nascent in vitro dental plaques by human host-defence peptides. FEMS Immunol. Med. Microbiol. 2011 6. doi.org/10.1111/j.1574-695X.2011.00922.x
Ogawa Y., Miura Y., Harazono A., Kanai-AzumaM., Akimoto Y., Kawakami H., Yamaguchi T., Toda T., Endo T, Tsubuki M., et al. Proteomic analysis of two types of exosomes in human whole saliva. Bioljgical and Pharmaceutical Bulletin. 2011;34:13–23. 11. Gorr S.-U.
Boman H.G. Antibacterial peptides: Basic facts andemerging concepts. J. Intern. Med. 2003;254:197–215. 76.
Melekhin V.V., Makeev O.G. Klotho gene: modernview on the structure and function. Possible mechanisms of anti-tumor action Immunologiya, mikrobiologiya, genetika. 2018, Том 15, №3, с. 393–404, DOI: 10.22138/2500- 0918-2018-15-3-393-404.
Imura A., Tsuji Y., Murata M., Maeda R., KubotaK., Iwano A. et al. α-Klotho as a regulator of calcium homeo-stasis //Science. – 2007. – Т. 316. – №. 5831. – С. 1615-1618. doi: 10.1126/science.1135901.
Arking D.E., Becker D.M., Yanek L.R., Fallin D.,Judge D.P., Moy T. F. et al. Klotho allele status and the risk of early-onset occult coronary artery disease. The American Jour-nal of Human Genetics. – 2003. – Т. 72. – №. 5. – С. 1154-1161. doi: 10.1086/375035.
