COMPARATIVE ANALYSIS OF THE SPECIES COMPOSITION OF DENTAL BIOFILM DEPENDING ON THE STATE OF THE HARD DENTAL TISSUES

Abstract

Purpose of the study. Identification of the species composition of the planktonic form of the dental biofilm in the cervical region of the vestibular surface of young patients’ teeth; carrying out a comparative analysis of obtained results in the patients with a wedge-shaped defect, cervical caries and clinically intact teeth. Materials and methods of research. The study involved 33 cariesresistant patients (average age 23.90±5.34 years) who were divided into three groups depending on the condition of the hard dental tissues. The material for microbiological research was the planktonic form of dental biofilm which was removed from the cervical region of the vestibular surface of clinically intact premolars. Group I had subgroup A, in which the species composition of dental biofilm taken from surfaces forming a wedge-shaped defect was studied. Isolated pure cultures of microorganisms were identified by morphological, tinctorial and biochemical characteristics. Scientific novelty. 72 strains of bacterial cultures, 11 fungi of six genera and ten species were isolated and identified. The taxonomic composition of the dental biofilm was represented by non-pathogenic and opportunistic microflora. In most cultures, these were gram-positive microorganisms, cocci, aerobes and facultative anaerobes. Bacteria of the genus Streptococcus dominated among cocci which were identified in 90.9±2.7% of the samples. In the patients with a wedgeshaped defect and intact teeth, the maximum frequency of excretion was γ-hemolytic streptococci, in the patients with cervical caries ‑ α-hemolytic streptococci which Str. mutans belongs to. More often, the dental biofilm was multicomponent and it contained fungi of Candida genus in 37.0% of the cases. The determined associations were mainly aerobic (55.6%). A greater number of the cases of association was determined in the patients with cervical caries.Conclusions. The obtained results are important for planning individual treatment and preventive measures in the patients with cervical pathology of the teeth. Further research of the dental biofilm, as well as understanding the impact of modern tools on it, is one of the urgent issues related to the dental health of young people.