INFLUENCE OF EXPERIMENTAL MODELING OF VIOLATION OF THE TIMING OF TEETHING ON MORPHOMETRIC PARAMETERS OF THE LOWER JAW AND FEMUR BONES OF RATS

Abstract

Experimental modeling of violation of the timing of teething is an important aspect of the study of pathological processes of the oral cavity. Purpose of the work. To study the causes of delayed teething and develop ways to correct this process that will allow you to choose effective methods for preventing concomitant pathology. Materials and methods. Experimental studies were performed on 20 white laboratory female rats and 37 pups that were born to them. Depending on the drugs used, the animals were divided into 4 Groups: 1. Intact (vivarium diet); 2. Vivarium diet + L-thyroxine at a dose of 10 mg/kg; 3. Vivarium diet + antibiotics (cefoperazone 180 mg/kg – pregnancy, amoxiclav 135 mg/kg – lactation); 4. Vivarium diet + Mercazolil – (20 mg/kg – pregnancy), (50 mg/kg – lactation). Further studies were conducted on rat pups that were born to females receiving various drugs. The groups were: 1. Intact (vivarium diet), n=7.2. vivarium diet + L-thyroxine, n=12.3. vivarium diet + antibiotics, n=11.4. vivarium diet + Mercazolil, n=7. Rats were removed from the experiment under thiopental anesthesia (20 mg/kg) after a lactation period of approximately 35 days. The total duration of the experiment was 56 days. Research results. Of the drugs used in this experiment, only mercazolil caused statistically likely changes in the parameters of the rat femurs, and these changes were negative for all the studied indicators. Thus, the average value of femoral density – 1.235±0.004 mg/mm3 – was lower than the density range in rats of other groups – 1.256±0.014 – 1.276±0.006 mg/mm3. At the same time, the femoral density in rats of this group was significantly lower than in rats from the groups with L-thyroxine and antibiotics (p<0.001), and was also 1.67% lower (p>0.05) than in rats of the Intact Group. Conclusions. 1. As a result of the conducted studies, low values of the density, mass and linear dimensions of the femur bones were established in rats with delayed teething born to females treated with mercazolil. 2. In the lower jaw of rats with delayed teething born to females treated with mercazolil, there was a tendency to reduce the density, total length of the lower jaw, length without incisor and jaw height compared to similar indicators in rats from other groups.