DYNAMICS OF CHANGES IN BIOCHEMICAL MARKERS IN THE ALVEOLAR PROCESS OF RATS UNDER THE INFLUENCE OF THE PROPOSED THERAPEUTIC AND PREVENTIVE COMPLEX IN THE CONDITIONS OF DEMENTIA SIMULATION

Abstract

Purpose of the study. Evaluation of the effect of the proposed complex of geroprotective, antioxidant, hepatoprotective, probiotic, anti-inflammatory effects on the state of the alveolar bone tissue of rats in the conditions of simulation of experimental dementia. Materials and methods of research. Experimental studies were conducted in accordance with the requirements of the law of Ukraine “on the protection of animals from ill- treatment” and the procedure for conducting experiments and experiments on animals by scientific institutions in compliance with bioethical standards. Aluminum intoxication of rats was chosen to simulate experimental dementia. The experiment was conducted on 24 male white rats, which were divided into 3 groups of 8 rats each: 1 group – intact animals; 2nd group – introduction of an aqueous solution of aluminum chloride (simulation of dementia); group 3 – modeling of dementia + application of the treatment and prevention complex. Research results and their discussion. The determination of biochemical markers in the alveolar homogenates of the jaws indicated a violation of the mineralization processes in the bone tissue when modeling dementia (a significant decrease in the alkaline phosphatase activity, an increase in the acid phosphatase activity, a decrease in calcium concentration). The increased activity of elastase indicated the intensification of the inflammatory process. A significant decrease in total protein in aluminum-intoxicated rats compared to intact rats indicated a disruption of the collagen matrix of bone tissue. Administration of the proposed prophylactic complex to rats of the 3rd group inhibited resorptive and inflammatory processes in the alveolar process of experimental animals, which was confirmed by the dynamics of enzyme activity changes. The content of calcium in the bone tissue of the jaws in rats of the main group was restored almost to the level of intact animals. The concentration of total protein increased, which indirectly indicates an increase in the amount of collagen in the bone tissue. Conclusions. It was established that long-term intoxication of rats with aluminum chloride leads to an increase in resorptive processes in the alveolar bone of experimental animals, as evidenced by the dynamics of changes in markers of bone metabolism (increase in the acid phosphatase activity, decrease in the alkaline phosphatase activity), a decrease in total protein, which indicates a violation of the collagen matrix of the bone fabrics The periodontoprotective effectiveness of the proposed treatment-prophylactic complex has been proven in the conditions of modeling dementia in rats due to intoxication with aluminum chloride, which is confirmed by the inhibition of resorptive processes in the alveolar process of the jaws of experimental animals.