DYNAMICS OF MARKERS OF SYSTEMIC INFLAMMATION, ANTIOXIDANT DEFENSE AND SOME BIOCHEMICAL PARAMETERS IN GUNSHOT WOUNDS OF THE ORAL MUCOSA IN THE EXPERIMENT (IN RATS)
DOI:
https://doi.org/10.35220/2523-420X/2024.1.5Keywords:
gunshot wounds, maxillofacial area, experimental study, biochemical parametersAbstract
Purpose of the study. Features of the course of gunshot lesions. In the course of the study, the following tasks were solved: in the obtained blood serum, determine the content of MDA and elastase activity (markers of systemic inflammation), catalase activity (a marker of antioxidant protection), and conduct a general blood test. In tissue homogenates, determine the level of biochemical markers of inflammation: elastase activity and the content of Malone dialaldehyde (MDA), as well as urease activity (an indicator of microbial contamination), catalase activity (an antioxidant enzyme), and acid (ACh) phosphatases. Alkaline (ALP) and acidic (ACP) phosphatase activity and elastase activity were determined in periodontal bone homogenates. Materials and methods of research. Experiments were conducted on White Rats of The Wistar line (males, 7 months, weight 400–450 g), which were divided into groups: intact – control; a group of animals with a non-hypnotic simulated fracture of the upper jaw on the left; a group of animals with a simulated gunshot wound to the upper jaw on the left (“experimental model of gunshot injuries to the jaws in rats” copyright No. 119858). The duration of the experiment was 28 days. Measurements of indicators in the experimental groups were carried out in 4 stages of 5 animals from the group: after 7 days of the experiment; after 14 days; after 21 days; after 28 days. Research results. The processes of inflammation (activity of elastase, acid phosphatase), contamination with opportunistic bacteria (urease activity), activation of lipid peroxidation (malondialdehyde content) against the background of reduced antioxidant protection (catalase activity) in the oral mucosa of rats after gunshot wounds were more intense than after jaw fracture. Normalization of the studied parameters after a non-gunshot fracture was carried out on day 21–28, and after gunshot wounds at the last stage in 28 days the activity of elastase, urease and catalase in the oral mucosa of rats did not correspond to the normal level, i.e. signs of inflammation, bacterial contamination and a decrease in antioxidant protection in the oral cavity of rats remained.
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