EXPERIMENTAL EVALUATION OF BIOCHEMICAL MARKERS OF THE ANTIOXIDANT-PROOXIDANT SYSTEM IN RAT GINGIVAL HOMOGENATES IN THE CONTEXT OF IMPLANT FIXATION AND THERAPEUTIC-PROPHYLACTIC MEASURES
DOI:
https://doi.org/10.35220/2523-420X/2024.2.1Keywords:
implants, gums, rats, experiment, biochemical markersAbstract
Recent studies in the field of dentistry have focused on the impact of oxidative stress on inflammatory processes after implant fixation, which can cause complications. Therefore, it is important to find new therapeutic approaches to minimize these negative effects and provide antioxidant protection. The purpose of the study was to evaluate biochemical markers of the antioxidant-prooxidant system in the gums of rats during implant fixation and the effect of treatment measures on these markers using an experiment. Materials and methods. The study involved 45 male rats of 7 months of age, with an average weight of 340–370 g. The animals of the experimental groups had their implants fixed, and the next day they were orally administered a complex of drugs; the control group was administered water in the same volume. The rats were divided into 3 groups. At the end of the experiment, a section of the gingival mucosa was separated for biochemical studies: the indicators of the antioxidant-prooxidant system were determined. A statistically significant difference between alternative quantitative features with a distribution corresponding to the normal law was evaluated using Student’s t-test. The difference was considered statistically significant at p<0.01. Research results. The data demonstrate that implant fixation causes significant changes in the gums of rats: the presence of inflammatory processes, intensification of lipid peroxidation processes, and a simultaneous decrease in the activity of antioxidant defense in gingival tissues. The use of the developed therapeutic complex of drugs against the background of implant fixation leads to the normalization of these indicators, which practically correspond to those of the intact group. Conclusions. The results obtained indicate the development of oxidative stress and inflammation in the gingival tissues after implantation. The use of a therapeutic complex of drugs effectively reduces oxidative stress and restores antioxidant protection, which indicates its potential effectiveness in the prevention and treatment of inflammatory complications after implantation, with the prospect of implementation in clinical practice.
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