STUDY OF THE EFFECT OF THE DEVELOPED THERAPEUTIC AND PREVENTIVE COMPLEXES ON THE BIOCHEMICAL INDICES OF THE ORAL MUCOSA AND BLOOD SERUM OF RATS WITH TEETHING DISORDER AGAINST THE BACKGROUND OF A CARIOGENIC DIET
DOI:
https://doi.org/10.35220/2523-420X/2024.3.1Keywords:
experiment, teething, biochemical indices of blood serum, biochemical indices of the mucous membrane of the oral cavityAbstract
Teething disorder can cause accompanying pathological changes in the tissues of the oral cavity; therefore it is important to conduct experimental studies of this effect, as well as to study the effectiveness of preventive measures that can be used in such patients. Objective: to study the effect of the developed therapeuticprophylactic complexes on the indices of urease and lysozyme of the mucous membrane of the oral cavity, alkaline phosphatase, elastase and catalase in the blood serum of rats with teething disorder against the background of a cariogenic diet. Methods. Rats were divided into 8 groups. The animals of each group were given a cariogenic diet. The 1st (control) group consisted of 8 intact rats. A group 2 consisted of 10 rats with early teething. The 3rd group included 10 rats with early teething that received therapeutic-prophylactic complex (TPC) No1. A group 4 consisted of 10 rats with delayed teething (born to females that received antibiotics during pregnancy and lactation). The 5th group consisted of 11 rats with delayed teething (born to females that received antibiotics during pregnancy and lactation), which received TPC No2. The 6th group comprised rats that were modelled delayed teething (born to females that received Mercazolil during pregnancy and lactation). The 7th group included rats with delayed teething (born to females that received Mercazolil during pregnancy and lactation and TPC No. 2. The activity of urease and lysozyme was determined in the homogenates of the mucous membrane of the oral cavity. The activity of alkaline phosphatase (AL), lysozyme, elastase and catalase was determined in the blood serum. Results. The use of TPC No. 1 in animals with early teething enabled to reduce the level of urease by 5.33 times and increase the level of lysozyme by 2.65 times in comparison with the 2nd group of rats. Catalase activity in the blood serum was 43.09% higher in rats of the group 5 that were treated with TPC No2 than in the animals of the group 4. In rats of the group 7, the level of catalase was higher by 50.82% than in animals of the group 6. Conclusions. As a result of the study, it was established that the use of the proposed therapeutic and preventive complexes in rats with teething disorders against the background of a cariogenic diet allows normalizing the microbiocenosis in the mucous membranes of the oral cavity, antioxidant protection and the level of non-specific immunoresistance in the blood serum.
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