EFFECT OF THE THERAPEUTIC AND PREVENTIVE COMPLEX ON THE CONDITION OF HARD TISSUES AND PULP OF RAT TEETH UNDER CONDITIONS OF CARIESCAUSING DIET AND ALIMENTARY VITAMIN D DEFICIENCY AGAINST THE BACKGROUND OF DESTRUCTION OF JAW BONE TISSUE

Abstract

Despite a number of studies, there is no single view on assessing the state of tooth pulp and periodontitis in destructive forms of periodontitis against the background of impaired bone metabolism. Vitamin D plays an important role in the pathogenesis of such widespread diseases of the bone system as osteoporosis and disorders of reparative osteogenesis. In this regard, the study of the role of various proteins and peptides in the implementation of the response of tooth pulp and periodontal to various pathogenic problems is particularly important for identifying the mechanisms of adaptation of these structures, both in inflammation and under the influence of iatrogenic factors. Understanding these mechanisms can lay the foundation for the development of new approaches to therapeutic and preventive actions in dentistry. The purpose of this paper. Experimental evaluation of changes in hard tissues and biochemical parameters of dental pulp in rats under conditions of caries-causing diet and alimentary vitamin D deficiency against the background of destruction of jaw bone tissue. Materials and methods of research. 30 male white rats of The Wistar line aged 4 months were used in experimental studies. The duration of the experiment is 33 days. 3 groups of 10 animals were formed: intact – rats without surgical manipulations and without Drug Administration, consumed a full-fledged combined feed; pathologytrauma to the lower jaw area in the projection of molar roots and modeling of alimentary hypovitaminosis D on the background of a high-sugar caries-causing diet; treatment – trauma to the lower jaw area in the projection of molar roots, introduction of a therapeutic and preventive complex against the background of modeling alimentary hypovitaminosis D using a high-sugar caries-causing diet. Aquadetrim vitamin D3, Osteovit, Mummy gel and Forteza spray are added to TPC. Injuries to the lower jaw were performed in a vivarium using a portable drill and sterile carbide boron under thiopental anesthesia. To study the influence of karyesogenic factors on the restoration of the injured jaw, the karyesogenic diet of M. S. Bugayova and S. A. Nikitin was used in the modification of I. V. Khodakov et al.for further research, jaws with teeth were isolated to count the number and depth of carious cavities; pulp, in which the activity of acid and alkaline phosphatases was determined. Research results. The consumption of a carious diet by rats in conditions of alimentary vitamin D3 deficiency against the background of bone destruction contributed to the development of the carious process. The caries-corrosive effect in Group 2 compared to the Intact Group was 86.6 ± 5.6. the use of the therapeutic and preventive complex could not completely slow down the development of the carious process in rats, while it had a pronounced positive effect on the impaired activity of pulp phosphatases. The mineralizing index of pulp decreased by 1.7 times. After applying the therapeutic and preventive complex, the mineralizing Index returned to the indicators of intact groups; the caries-prophylactic effect in animals of Group 3 was 39.28% (p1 < 0.001); a positive effect on the impaired activity of pulp phosphatases was obtained. Conclusions. As a result of experimental studies, the use of TPC could not completely slow down the development of the carious process in rats. At the same time, the preventive effect of TPC on the activity of pulp phosphatases under the specified conditions was established.