POLYMORPHISM OF VDR BSM G>A, COL1A1 1546 SP1,G>T, MMP9 A8202G, CALCR C1377T, CASR A986S GENES IN CHILDREN WITH DENTAL PATHOLOGY ON THE BACKGROUND OF DISEASES OF THE MUSCULOSKOLE SYSTEM AND VITAMIN D DEFICIENCY

Abstract

Basic studies have confirmed that dental caries in children is accompanied by local and systemic disorders of calcium-phosphorus metabolism and is a visible marker of immune disorders and the state of bone tissue of the body as a whole. Lack of vitamin D causes problems with enamel, which directly provides increased caries. Insufficient intake of calcium, phosphorus, magnesium and vitamin D in the child's body, especially during the growth spurt period, can negatively affect the formation of the bone matrix. Connective tissue performs numerous and important functions in the body, responding to almost all pathological and physiological influences. Therefore, the quality of metabolic processes occurring in it depends on the processes of adaptation of the body and the stability of its organs and systems, including such important ones as the musculoskeletal system. In this regard, there is a growing interest in studying genetic factors that can influence the predisposition, development and individual severity of dental diseases, especially in patients with musculoskeletal disorders. The aim of this study was to evaluate the effect of polymorphic variants of the VDR BSMG>a, SALCR C1377T ,CASR A986S genes on the development of vitamin D deficiency and osteoporosis, which accompanies most musculoskeletal systemdiseases in children. Materials and methods of research. During the study, the genetic polymorphism of VDR BSMG>a ,SALCR C1377T, CASR a986s genes was studied in groups of patients with dental pathology against the background of vitamin D deficiency, osteoporosis against the background of diseases of the musculoskeletal system and the control group for the purpose of personalized preventive and curative dental measures. The a986s polymorphism of the CASR gene (rs1801725) was evaluated by three- primer PCR with two direct primers for the normal and mutant gene variants and one common Reverse Primer. The CALCR c1377t gene polymorphism (rs1801197) was determined by PCR-PDFR with appropriate primers. The frequency of BSMI polymorphism of the VDR 283A/G gene (BsmI) (rs1544410) was evaluated by PCR with real- time detection of results and analysis of melting curves. Research results and their discussion. A comparative analysis of different genotypes of the BSM polymorphic marker of the VDR gene showed that the GG genotype is more common – 50% in children with osteoporosis. The frequency of the G allele in children with osteoporosis was 70%. Ga heterozygote was most common in children in the control group and with vitamin D deficiency (60%). The frequency of the G allele in children with osteoporosis was 70%. Homozygous (TT) polymorphism of the SALCR c1377t gene was found in 70% of children with vitamin D deficiency. Among children with vitamin D deficiency, the T allele was 20% more likely to be detected compared to children in the control group and children with osteoporosis. Only in the GS genotype of the CASR A986S gene, there is an average strength of association between the risk factor and the incidence of op (p < 0.001). Conclusions. There is no reliable association between the dependence of the D-deficient state on the BSM genotype of the VDR polymorphic marker. The frequencies of alleles and genotypes of the BSM polymorphism of the VDR gene in children with OP and D-deficient conditions do not have statistically significant differences from the control group of children. When evaluating the CaSR a986s gene polymorphism, no significant associations were obtained with the presence of OP and vitamin D deficiency. The study of the CASR a986s gene as a marker of increased risk of OP and vitamin D deficiency is not appropriate. We can conclude that the reason for the high prevalence of vitamin D deficiency lies in its insufficient intake from food and reduced endogenous synthesis in the skin under the influence of ultraviolet radiation.