REPLACEMENT OF LOWER JAW DEFECTS IN RATS BY DECELULARIZED, LYOPHILIZED AND RECELULARIZED BONE SCAFFOLD

Abstract

This work concerns to the possibility of application of decelularized, lyophilized and recelularized bone scaffold for large defects replacement of flat facial bones and bones of the skull as well. The studies were performed on Lewis inbred rats. The bone matrix was obtained from donor rats. After decelularisation and lyophilization bone matrix was recelularized with bone marrow derived stem cells. Post operative studies have shown, that the lower jaw bone defect was completely filled with the newly formed bone tissue with large number of full-fledged blood vessels by the end of three weeks. At later time points (one month), the bone defect was completely obscured by the newly formed bone tissue. Thus we can conclude that the healing of bone injuries, characteristics and rate of regenerative process is directly related to osteoinductive and osteoconductive potency of biological material used in the experiment. The decelularized, lyophilized bone scaffold recelularized with bone marrow stem cells maight serve as a biomaterial. The aim of the study is to determine the possibility of the application of decellulased, frozen-dried and recellulased bone matrix from allotissue of lower jaw as the material for the replacement of defects in jaw. The experimental studies have shown, that the healing of bone wound, peculiarities and the speed of the formation and the maturation of bone regenerate is connected directly to the osteoinductive and osteoconductive potential of the biomaterial, used in the experiment. The initial studies have shown, that decellulased, frozen-dried and recellulased bone matrix can be used for the surgical treatment of extensive defects of not only flat bones of facial skeleton, but also the bones of calvarium in whole.