FEATURES OF THE ANTIOXIDANT- PROOXIDANT SYSTEM AND INFLAMMATORY MARKER IN THE BLOOD SERUM OF RATS AGAINST THE BACKGROUND OF CHRONIC STRESS MODELLING AND TREATMENT AND PREVENTION MEASURES
DOI:
https://doi.org/10.35220/2523-420X/2025.1.16Keywords:
stress, carious process, blood serum, rats, experimentAbstract
Chronic psycho-emotional stress provokes a persistent imbalance between antioxidant and pro-oxidant processes, leading to cellular damage and systemic inflammation. Optimising pharmacological correction of these disturbances remains a pressing issue in experimental and clinical medicine. Aim of the study.To evaluate the effect of a therapeutic complex of drugs on the parameters of the antioxidant-prooxidant system and inflammatory markers in the blood serum of rats under chronic stress modeling. Materials and methods.The study included 34 male Wistar rats (2 months old, body weight 140 ± 8 g), which were divided into three groups: an intact group (n = 10), a group with induced chronic sound stress (n = 12), and a group with induced stress followed by administration of a therapeutic- prophylactic complex (TPC) (n = 12). Over 50 days, the rats in the second and third groups were exposed to chronic sound stress using an ultrasonic pest repellent.In the “stress + TPC” group, the rats additionally received the therapeutic-prophylactic complex. Upon completion of the experiment, biochemical parameters of blood serum, were determined. Statistical processing was performed using STATISTICA 6.1 with Student’s t-test (p < 0.01 was considered significant). Results. Stress increased elastase activity and MDA by 26.3 % and 64.2 % respectively, reduced catalase activity by 23.1 % and decreased API 4.4- fold versus intact animals. TPC administration lowered elastase activity by 14.8 %, reduced MDA by 41.3 %, increased catalase activity by 20.0 % and restored API to near-control values. Conclusions. Chronic acoustic stress induces pronounced oxidative imbalance and inflammation in rats. The tested TPC exhibits significant antioxidant and anti-inflammatory efficacy, restoring the functional equilibrium of the antioxidant–lipid peroxidation system under stress conditions.
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