CORRECTION OF BONE RESORPTION PARAMETERS IN RATS INDUCED BY MODELLING CHRONIC STRESS BY A THERAPEUTIC-PROPHYLACTIC COMPLEX

Abstract

Chronic psycho-emotional stress disrupts bone remodelling, leading to enhanced resorption and suppressed osteogenesis. Comprehensive pharmacological strategies possessing antioxidant and membrane-stabilising properties remain insufficiently explored as means of correcting stress-induced skeletal alterations. Aim of the study. To evaluate the efficacy of a therapeutic-prophylactic complex in normalising bone resorption parameters in rats subjected to a model of chronic acoustic stress. Materials and methods. The study included 34 male Wistar rats (2 months old, body weight 140±8 g), which were divided into three groups: an intact group (n=10), a group with induced chronic sound stress (n=12), and a group with induced stress followed by administration of a therapeutic-prophylactic complex (TPC) (n=12). Over 50 days, the rats in the second and third groups were exposed to chronic sound stress using an ultrasonic pest repellent. In the “stress + TPC” group, the rats additionally received the therapeuticprophylactic complex. In mandibular homogenates, the activities of acid phosphatase (AP), alkaline phosphatase (ALP) and the mineralisation index (ALP/AP) were determined. Statistical processing was performed using STATISTICA 6.1 with Student’s t-test (p<0.01 was considered significant). Results. Chronic stress increased AP activity by 23.7% and reduced ALP activity by 33.0% versus intact controls, resulting in a 51.3% decline in the mineralisation index. TPC administration lowered AP activity by 31.2% and elevated ALP activity by 19.5% relative to the stress group, restoring the mineralisation index to 76% of the control value. Conclusions. The therapeutic-prophylactic drug complex effectively corrects stress-induced disturbances in bone remodelling by attenuating resorption and reinstating osteogenic activity. These findings support the prophylactic use of TPC to mitigate bone complications associated with chronic stress.