MORPHOLOGICAL CHANGES IN THE WOUND PROCESS AFTER IMPLANTATION OF SILVER ALLOY PIERCINGS IN LABORATORY ANIMALS
DOI:
https://doi.org/10.35220/2523-420X/2025.3.7Keywords:
piercing, silver alloy, experiment, granulation tissue, inflammationAbstract
Purpose of the study. To prove the effect of selenium alloy piercing on the course of the wound process in the soft tissues of the oral cavity through morphological studies of microdissections in experimental animals. Materials and methods. Experimental studies were conducted on mature rabbits of the Chinchilla breed weighing from 2.7 to 3.2 kg. The procedures were in accordance with the European Convention for the Protection of Vertebrate Animals (Strasbourg, March 18, 1986), EU Council Directives (November 24, 1986) and Protocol No. 6 of the KhNMU Ethics Committee of June 5, 2013. Implantation of a silver alloy piercing into the soft tissues of the tongue and cheek. Histologic analysis of changes in the wound channel was performed on days 7, 14, 28, and 60. Morphological studies were performed at the Central Research Laboratory and at the Department of Pathological Anatomy of KhNMU. The material was fixed in 10% formalin, treated with alcohol, and embedded in paraffin, sections 4-5 μm. Analysis and photographs were taken with an Olympus BX-41 microscope (Japan). Scientific novelty. On the 7th day, purulent-necrotic detritus, initial epithelialization at the edges, granulation tissue with capillaries, fibroblasts and an infiltrate of lymphocytes and neutrophils were observed in the wounds of the tongue and cheek. On the 14th day, the epithelium is thickened with hyper- and parakeratosis, acanthosis, and microabscesses; granulation tissue matures, collagen fibers are bundled, and cellular elements are represented by fibroblasts, macrophages, and neutrophils. On day 28, epithelialization is uneven, with microabscesses and inflammation; granulation tissue matures, with young connective tissue in the deeper parts. On day 60, the epithelium is uneven, sometimes thickened or thinned; granulation tissue is voluminous, with bundled collagen fibers and secondary granulations inside and on the surface of the epithelial layer. Conclusions. The healing of the wound channels of the tongue and cheek after implantation of silver piercing clips is accompanied by inflammation and repair. On days 7-14, purulentnecrotic detritus, neutrophil infiltration, granulation tissue formation, and the onset of epithelialization dominate. On day 28-60, epithelialization is uneven with microabscesses and necrosis, indicating chronicity of the process. The granulation tissue matures, but secondary granulations make recovery difficult. This indicates long-term irritation from silver clips and requires caution when choosing piercing materials.
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