EXPERIMENTAL EVALUATION OF MINERALIZATION INDICATORS IN RAT ORAL FLUID UNDER CHRONIC STRESS MODELING AND THERAPEUTIC AND PREVENTIVE MEASURES

Abstract

Prolonged psychoemotional impact, particularly sound stress, plays a significant role in the development of various pathological processes in the dentoalveolar system. The negative effect of stress is often manifested through oxidative tissue damage, which may increase the risk of caries-related changes and accelerate the resorption of periodontal bone tissue. At the same time, the search for effective therapeutic and prophylactic complexes capable of compensating for the destructive impact of stress remains a pressing issue. Aim of the study. To evaluate the effect of a therapeutic complex of drugs on the carious process and the degree of alveolar bone resorption in the jaws of rats under chronic stress modeling. Materials and methods. The study included 34 male Wistar rats (2 months old, body weight 140±8 g), which were divided into three groups: an intact group (n=10), a group with induced chronic sound stress (n=12), and a group with induced stress followed by administration of a therapeuticprophylactic complex (TPC) (n=12). Over 50 days, the rats in the second and third groups were exposed to chronic sound stress using an ultrasonic pest repellent. In the “stress + TPC” group, the rats additionally received the therapeutic-prophylactic complex. Upon completion of the experiment, the number and depth of carious cavities, as well as the degree of alveolar ridge resorption, were determined. Statistical processing was performed using STATISTICA 6.1 with Student’s t-test (p<0.01 was considered significant). Results. Rats exposed to chronic sound stress showed a statistically significant increase in the intensity of carious lesions and enhanced resorptive processes in the bone tissue compared to the intact group. Meanwhile, the administration of the therapeuticprophylactic complex under prolonged sound stress conditions substantially improved the morphofunctional parameters of the dentoalveolar system: both the number and depth of carious lesions decreased, and the destruction of the alveolar ridge slowed down. Conclusions. The obtained results indicate a pronounced caries-preventive and periodontoprotective effect of the tested drug composition under conditions of sound stress. The use of this complex may be a promising approach in developing preventive and therapeutic strategies aimed at averting and correcting stress-induced caries-related changes in the dentoalveolar system.