CORRECTION OF BIOCHEMICAL MARKERS OF INFLAMMATION AND DYSBIOSIS IN THE ORAL MUCOSA OF RATS EXPOSED TO STRESS MODELLING USING ATHERAPEUTIC AND PROPHYLACTIC COMPLEX

Abstract

Chronic acoustic stress provokes oxidative–inflammatory shifts and disrupts the microbiocenosis of the oral mucosa, creating prerequisites for the development of dental pathologies. The pharmacological correction of these disturbances with multicomponent therapeutic– prophylactic agents remains an important task of experimental dentistry. Aim of the study. To determine the effectiveness of a therapeutic-prophylactic complex (TPC) in correcting biochemical indicators of inflammation and dysbiosis in the oral mucosa of rats under chronic acoustic stress. Materials and methods. The study included 27 male Wistar rats (2 months old), divided into three groups (9 animals in each): an intact group (Group 1), a group exposed to acoustic stress (Group 2), and a group with acoustic stress plus additional administration of a therapeutic-prophylactic complex (Group 3). The experiment lasted for 49 days. Stress was modeled using an ultrasonic repeller (30–65 kHz), operated 6 hours daily, with the combined addition of sound in the audible range. Animals in Group 3 received the therapeutic-prophylactic agents orally. Acid phosphatase (AP), elastase, lysozyme, urease activities and malondialdehyde (MDH) content were determined in the oral mucosa. Statistical analysis was performed using Student’s t-test (p<0.01). Results. Chronic acoustic stress significantly increased AP activity by 65%, elastase by 77% and MDH by 63% compared with intact animals; lysozyme activity decreased by 36%, while urease activity rose by 50%. TPC administration reduced AP by 33%, elastase by 30% and MDH by 23% relative to the stress control, approaching baseline values. Lysozyme activity rose by 23%, and urease activity fell by 19% compared with untreated stressed rats. Conclusions. The multicomponent TPC effectively mitigates oxidative– inflammatory changes and dysbiosis of the oral mucosa induced by chronic acoustic stress, as evidenced by normalisation of AP, elastase, MDH, lysozyme and urease. These findings highlight the potential of comprehensive pharmacological prophylaxis for stress-associated dental lesions.